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John Chapman packed a horse load of apple seed from Pennsylvania cider presses into the Territory of Ohio in 1801, according to a magazine article written in 1871. He planted his first orchard** there near Licking Creek. According to the author, Chapman was again spotted carrying apple seeds to the frontier by way of the Ohio River in 1806:
It was "Johnny Appleseed," by which name Jonathan Chapman was afterward known in every log cabin from the Ohio River to the Northern lakes, and westward to the prairies of what is now the state of Indiana.
This article is unique because it is from a per- spective of only 70 years removed from Chapman’s
maiden travels into the wilderness—only 26 years removed from the time of John Chapman's death.
Fortunately, a readable photocopy of the Harper’s New Monthly Magazine article has been published on the Net.

**According to a Sheffield, Pennsylvania, newspaper article, Johnny's first nursery was in Warren County, Pennsylvania, about 1796. Specifically, it was located at Brokenstraw Creek.

source: http://www.appleappetite.com/Johnny.htm

I found this information posted on the link listed above.